Bioaerosols

Bioaerosols are airborne particles containing biological material. Their occupational hazards must include the source, the tasks that generate them, and potential infectious, allergic, or toxic effects.

In short

A bioaerosol can contain microorganisms, cell fragments, or other biological materials. Controlling its generation and dispersion is essential, even if a measurement does not detect culturable microorganisms.

Content
  1. What are bioaerosols?
  2. Where exposure can occur
  3. How to approach the evaluation
  4. What do measurements contribute and what are their limitations?
  5. Control the source and the spread
  6. Staff protection and information
  7. Practical example
  8. Monitoring and common errors
  9. Related concepts
  10. On the blog
  11. References

AZ Dictionary →

What are bioaerosols?

The term bioaerosol describes biological material transported by air in particles or droplets. It can include bacteria, fungal spores, fragments of microorganisms, pollen, and other components of animal or plant origin. Its composition varies with the source, the mechanism of generation, and environmental conditions; it is not a single substance with constant behavior.

Their preventive relevance does not depend solely on the microorganisms being alive. Some components can produce allergic or toxic effects. Therefore, only looking for infection or using a single microbiological count may overlook part of the problem. The classification of biological agents must be interpreted within this broader context.

Where exposure can occur

Exposures can occur during waste and water treatment, the handling of organic materials, certain agricultural activities, and some healthcare procedures. It is also advisable to investigate sources related to humidity and biological contamination in buildings. The presence of an activity in this list does not necessarily prove a specific exposure; it is necessary to observe its actual conditions.

Operations that agitate materials or generate spray require special attention. Cleaning, unloading, or repairs can alter exposure compared to normal operations. The assessment should ask when the release occurs, how long it lasts, where people are located, and what systems prevent the material from reaching their breathing zone.

How to approach the evaluation

Industrial hygiene begins by characterizing the process and potential sources of contamination. Materials, humidity, ventilation, procedures, maintenance, and incident history should all be reviewed. Observations from those performing the task help identify exposure peaks that may not appear in an administrative job description.

Before sampling, it’s advisable to formulate the decision you intend to support: locating a source, comparing situations, studying a task, or verifying an improvement. Each question may require a different strategy. If an obvious source can be corrected immediately, an analytical campaign should not become an excuse to postpone that intervention.

What do measurements contribute and what are their limitations?

The sampling and analysis method determines what portion of the bioaerosol is observed. A culture provides information on the microorganisms recoverable under the conditions used; it does not represent all the biological material present. Time, flow rate, support, transport, and analytical technique all influence the results and must be documented.

Results vary across locations and time. A small sample may not capture an occasional occurrence, and an overloaded sample can hinder interpretation. In Spain, there is no general set of occupational exposure limit values ​​for biological agents equivalent to those used for many chemical contaminants. A single number does not guarantee workplace safety.

Control the source and the spread

Measures must be tailored to the identified source. These may include enclosing operations, proper extraction, maintenance, removal of accumulated waste, and cleaning procedures that reduce dispersion. The choice of measures must consider additional risks: disinfection or a product change may introduce chemical exposure and requires its own assessment.

It is useful to define observable operating conditions: status of closures, availability of extraction, material handling, and response to leaks. Preventive control does not end with the installation of equipment. Its maintenance procedures, failure indicators, and who is authorized to suspend operations when a barrier malfunctions must be clearly defined.

Staff protection and information

When necessary, respiratory protection is selected according to exposure and working conditions, with training and verification of its use. It should not automatically replace collective controls. Other equipment may be required for protection against splashes or contact, especially during cleaning and maintenance tasks.

The information should cover hygiene, clothing and equipment procedures, waste management, and incident reporting. Health surveillance is guided by identified risks. If symptoms or clusters of cases appear, a professional assessment is required; attributing them to the building or the process without investigation can lead to erroneous conclusions.

Practical example

Visible emissions are observed during unloading in an organic material sorting area. The safety team records the conditions of each operation, the position of personnel, and the state of the enclosures. They decide to improve the collection system and reorganize access before using the measurements as the sole criterion for action.

The subsequent strategy compares documented tasks and conditions, including peak emission periods. It verifies that the improvement does not shift emissions to another area or complicate cleanup. The report distinguishes between measured data, analytical limitations, and functional checks. This allows for informed decision-making without presenting the result as an absolute guarantee of risk-free operation.

Monitoring and common errors

Useful indicators include containment incidents, pending maintenance, operations that generate dispersion, and compliance with agreed-upon measures. These should be interpreted in conjunction with available technical and health information. If materials or processes change, previous exposure cannot be considered representative without review.

Common errors include equating the absence of growth in cultures with the absence of bioaerosols, comparing incompatible methods, and extrapolating from a single sample to the entire workday. Recommending a generic mask without studying the specific task is also insufficient. Prevention is improved when the source, route of exposure, and controls are linked in a reviewable assessment.

Related concepts

On the blog

References

  1. National Institute for Occupational Safety and Health. NTP 1064: Indoor air quality. Biological contaminants, sampling strategy. 2015. Official source
  2. National Institute for Occupational Safety and Health. NTP 608: Biological agents, measurement planning. 2001. Methodological reference. Official source
  3. National Institute for Occupational Safety and Health. Frequently Asked Technical Questions on Biological Hazards. Official Source
  4. National Institute for Occupational Safety and Health. Biological Risk Management. Official Source
  5. Official State Gazette. Royal Decree 664/1997, on protection against biological agents at work. Consolidated text. Official source

Editorial information

Publication date: October 10, 2026.

Editorial Manager: Sabentis Editorial Team.

Author: Pablo Rodríguez LinkedIn

Executive Vice President of the ORP International Foundation and Chief Financial Officer of Sabentis.

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